Journal: Frontiers in Physiology

Article Title: Impact of Bilateral Sympathetic Stellate Ganglionectomy on TGF-β1 Signaling Pathway in Rats With Chronic Volume Overload

doi: 10.3389/fphys.2020.00375

Figure Lengend Snippet: Expressions of norepinephrine (NE) and acetylcholine (ACh) in the plasma, right ventricle (RV), and left ventricle (LV) by ELISA. (A) The expression of NE was significantly increased in the abdominal aorta-cava fistula (AV) group and decreased in the bilateral sympathetic stellate ganglionectomy after abdominal aorta-cava fistula (AD) group in the plasma, RV, and LV. (B) The expression of ACh was significantly decreased in the AV group and increased in the AD group in the RV (* P < 0.05, ** P < 0.01) (three replicate experiments were performed).

Article Snippet: Acetylcholine (ACh) concentrations of the plasma, RV, and LV were determined with the ELISA kit (BioVision, E4454-100, Milpitas, CA, United States) following the manufacturer’s instructions.

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Expressing

Journal: Cell Death & Disease

Article Title: Bevacizumab attenuates osteosarcoma angiogenesis by suppressing MIAT encapsulated by serum-derived extracellular vesicles and facilitating miR-613-mediated GPR158 inhibition

doi: 10.1038/s41419-022-04620-3

Figure Lengend Snippet: A Proliferation of U2OS and MG63 cells treated with Bev at concentrations of 1, 5, and 25 mg/mL measured by CCK-8 assay. B Angiogenesis of Bev-treated U2OS and MG63 cells measured by microtubule formation in vitro. C ELISA detection of levels of angiogenesis-related genes HGF, VEGF-D, VEGF-C, VEGF, and ANGPTL4 in the supernatant of Bev-treated U2OS and MG63 cells. D Tumor volume of mice treated with Bev. E MVD in tumor tissues of mice treated with Bev was detected by IHC (scale bar: 25 μm). * p < 0.05, compared with control cells; ** p < 0.01, compared with control mice. n = 6 for mice upon each treatment. All experiments were conducted three times independently.

Article Snippet: ELISA kits (Abcam, Cambridge, UK) were used to detect the levels of HGF (ab100534), VEGF-D (ab233625), VEGF-C (ab9546), VEGF (ab100662), and ANGPTL4 (ab99974).

Techniques: CCK-8 Assay, In Vitro, Enzyme-linked Immunosorbent Assay, Control

Journal: Nature Communications

Article Title: Organ-specific genome diversity of replication-competent SARS-CoV-2

doi: 10.1038/s41467-021-26884-7

Figure Lengend Snippet: A total of 516 SARS-CoV-2 nucleocapsid protein (NP)-positive cells were evaluated for marker expression (123 for ACE2, 133 for cytokeratin [CK], 136 for CD14, and 120 for ICAM) across different organs of three cases with viral dissemination (case 06, 07, and 13). The first level of the sunburst chart represents the distribution of SARS-CoV-2 NP-positive cells across different organs (blue = lung, red = heart, brown = liver, green = kidney, purple = spleen). The second level of the sunburst chart depicts the percentage of cell types positive for SARS-CoV-2 NP per organ (forward slash = cytokeratin [CK], backward slash = CD14, crosshatch = ICAM, dotted = not identified [NI]), and the third level shows co-localization data with ACE2 (long and short form). Source data are provided as a Source Data file. Representative confocal images are grouped per organ (different colors) at the outer edges. Scale bars represent 25 µm.

Article Snippet: ACE2 and TMPRSS2 levels were quantified using the human ACE2 ELISA Kit (#ab235649; Abcam, Cambridge, United Kingdom) and human TMPRSS2 ELISA Kit (#NBP2-89171; Novus Biologicals, Centennial, CO, USA), respectively, according to the manufacturers’ instructions.

Techniques: Marker, Expressing

Journal: Redox Biology

Article Title: LPS-induced mitochondrial dysfunction regulates innate immunity activation and α-synuclein oligomerization in Parkinson's disease

doi: 10.1016/j.redox.2023.102714

Figure Lengend Snippet: LPS-induced mitochondrial dysfunction activates innate immunity. Primary mice mesencephalic neurons treated with LPS. (a) Representative immunoblot for TLR4 levels. (b) Densitometric analysis of TLR4. (n = 5; *p < 0.05, relatively to untreated mesencephalic neurons). (c) NF-κB levels. Values are in μg/mg protein. (n = 6; ***p < 0.001, relatively to untreated mesencephalic neurons). (d) Caspase-1 activation. (n = 6; **p < 0.01, relatively to untreated mesencephalic neurons). (e) Cytosolic IL-1β levels. (n = 6; **p < 0.01, relatively to untreated mesencephalic neurons). CT and PD cybrids exposed to LPS. (f) Representative immunoblot for TLR3 levels. (g) Densitometric analysis of TLR3. (n = 4; *p < 0.05, relatively to untreated CT cybrids). (h) Representative immunoblot of nuclear p65 NFκB. (i) Densitometric analysis of nuclear p65 NFκB. (n = 5; *p < 0.05, relatively to untreated CT cybrids). (j) Caspase-1 activation. (n = 6; *p < 0.05 and **p < 0.01, relatively to untreated CT cybrids). (k) Cytosolic IL-1β levels. (n = 6; CT cybrid Unt vs CT cybrid LPS, p = 0.057). NT2 Rho+ and Rho0 cells were treated with LPS. (l) Representative immunoblot for TLR3 levels. (m) Densitometric analysis of TLR3. (n = 4; *p < 0.05 and **p < 0.01, relatively to untreated NT2-Rho+). (n) Representative immunoblot of nuclear p65 NFκB. (o) Densitometric analysis of nuclear p65 NFκB. (n = 3; *p < 0.05, relatively to untreated NT2 Rho+). (p) Caspase-1 activation. (n = 6; *p < 0.05, relatively to untreated NT2-Rho+). (q) Cytosolic IL-1β levels. (n = 4; *p < 0.05, relatively to untreated NT2-Rho+).

Article Snippet: IL-1β and NF-κB levels were evaluated in 25 μg of cellular or neuronal extracts using Quantikine ELISA (R&D Systems; Cat. No. MLB00C) and NFκB p65 Total SimpleStep ELISA Kit (Abcam; Cat. No. ab176648), respectively, according to manufacturer's instructions.

Techniques: Western Blot, Activation Assay

Journal: Redox Biology

Article Title: LPS-induced mitochondrial dysfunction regulates innate immunity activation and α-synuclein oligomerization in Parkinson's disease

doi: 10.1016/j.redox.2023.102714

Figure Lengend Snippet:

Article Snippet: IL-1β and NF-κB levels were evaluated in 25 μg of cellular or neuronal extracts using Quantikine ELISA (R&D Systems; Cat. No. MLB00C) and NFκB p65 Total SimpleStep ELISA Kit (Abcam; Cat. No. ab176648), respectively, according to manufacturer's instructions.

Techniques: Enzyme-linked Immunosorbent Assay